19+ neb q5 tm calculator
Amplification of a variety of human genomic amplicons. The Q5 Hot Start High-Fidelity 2X Master Mix features a high-fidelity thermostable hot start DNA polymerase with 3 5 exonuclease activity fused to a processivity-enhancing Sso7d.
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Browse NEBs 18 interactive tools including Double Digest Finder.
. Q5 Blood Direct 2X Master Mix enables robust amplification direct from whole or dried blood PCR was performed using Q5 Blood Direct 2X Master Mix under. To determine the optimal annealing temperatures for a given set of primers use of the NEB Tm Calculator is highly recommended. The NEB Tm calculator is intended for use in estimating the optimal annealing temperature for PCR with NEB polymerases Tm values are calculated using thermodynamic data from Santa.
NEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the Q5 Site. NEBs Tm Calculator Figure 1. For POST requests the input is expected to be in serialized JSON format including an HTTP content-type header.
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